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More than 50% of Clostridium difficile Isolates from Pet Dogs in Flagstaff, USA, Carry Toxigenic Genotypes

By Nathan E. Stone, Lindsay C. Sidak-Loftis, Jason W. Sahl, Adam J. Vazquez, Kristin B. Wiggins, John D. Gillece, Nathan D. Hicks, James M. Schupp, Joseph D. Busch, Paul Keim, David M. Wagner

Category Journal Articles
Abstract

Nosocomial acquisition of Clostridium difficile is well documented, yet recent studies have highlighted the importance of community acquired infections and identified community associated reservoirs for this pathogen. Multiple studies have implicated companion pets and farm animals as possible sources of community acquired Cdifficile infections in humans. To explore the potential role of pet dogs in human Cdifficile infections we systematically collected canine fecal samples (n = 197) in Flagstaff, AZ. Additionally, nineteen fecal samples were collected at a local veterinary clinic from diarrheic dogs. We used these combined samples to investigate important questions regarding Cdifficile colonization in pet canines: 1) What is the prevalence and diversity of Cdifficile in this companion pet population, and 2) Do Cdifficileisolates collected from canines genetically overlap with isolates that cause disease in humans? We used a two-step sequence typing approach, including multilocus sequence typing to determine the overall genetic diversity of Cdifficile present in Flagstaff canines, and whole-genome sequencing to assess the fine-scale diversity patterns within identical multilocus sequence types from isolates obtained within and among multiple canine hosts. We detected Cdifficile in 17% of the canine fecal samples with 10% containing toxigenic strains that are known to cause human disease. Sequencing analyses revealed similar genotypes in dogs and humans. These findings suggest that companion pets are a potential source of community acquired Cdifficile infections in humans.

Submitter

Marcy Wilhelm-South

Purdue University

Publication Title PLoS One
Volume 11
Issue 10
Pages 21
DOI 10.1371/journal.pone.0164504
URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0164504
Cite this work

Researchers should cite this work as follows:

  • Nathan E. Stone; Lindsay C. Sidak-Loftis; Jason W. Sahl; Adam J. Vazquez; Kristin B. Wiggins; John D. Gillece; Nathan D. Hicks; James M. Schupp; Joseph D. Busch; Paul Keim; David M. Wagner (2018), "More than 50% of Clostridium difficile Isolates from Pet Dogs in Flagstaff, USA, Carry Toxigenic Genotypes," http://habricentral.org/resources/63976.

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Tags
  1. Analysis
  2. Animal roles
  3. Bacteria
  4. DNA
  5. Dogs
  6. Mammals
  7. open access
  8. Pets and companion animals
  9. United States of America
Badges
  1. open access