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Simple cannulation procedure for serial blood sampling through cutaneous ulnar vein in chickens

By D. M. Bayer, K. Mohan, K. Jayakumar, M. Manafi, B. H. Pavithra

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Abstract

The objective of the study was to collect repeated, low-stress blood samples from the ulnar vein of chickens required for pharmacokinetic studies or hormonal assays. The study used 5 apparently healthy, unsexed, commercial broiler chickens about 6 weeks old and weighing 1.7-1.9 kg for serial sampling of blood. The study prepared the birds prior to cannulation and penetrated the catheter through the skin and into the lumen of the ulnar vein. The study successfully carried out serial blood samplings in 4 of 5 cannulated birds. Heparin (10%) solution maintained patency and prevented blood clot formation inside the cannula. However, the study found repeated clotting occurring in 1 bird. Cannula failed to maintain patency; the study could not carry out blood sampling properly, which was attributed to air embolism that might have occurred during catheter manipulation or repeated filling of cannula with heparin solution. The study observed no hematoma or inflammation at the site of cannulation. Owing to the advantages and to facilitate compliance with nonhuman animal welfare, this technique seems simple and efficient, allowing adoption for serial blood collection in chickens.

Publication Title Journal of Applied Animal Welfare Science
Volume 15
Issue 1
Pages 91-100
ISBN/ISSN 1088-8705
Publisher Taylor & Francis
DOI 10.1080/10888705.2012.624925
Language English
Author Address Veterinary Faculty Clinics Department, Nangarhar University, Jalalabad, Afghanistan.
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Tags
  1. Animal diseases
  2. Animal rights
  3. Animals
  4. Animal welfare
  5. Birds
  6. Blood
  7. Broilers
  8. Chickens
  9. Fowls
  10. Hematoma
  11. Inflammation
  12. Meat animals
  13. peer-reviewed
  14. pharmacokinetics
  15. Pharmacology & Pharmacy
  16. Poultry
  17. Research
  18. sampling
  19. Studies
  20. Techniques
  21. toxicology
  22. vertebrates
Badges
  1. peer-reviewed