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Raw pet food as a risk factor for shedding of extended-spectrum beta-lactamaseproducing Enterobacteriaceae in household cats

By Valérie O. Baede, Els M. Broens, Mirlin P. Spaninks, Arjen J. Timmerman, Haitske Graveland, Jaap A. Wagenaar, Birgitta Duim, Joost Hordijk

Category Journal Articles
Abstract

Background
Close contact between pets and owners provides the opportunity for transmission of antimicrobial resistant organisms like extended-spectrum beta-lactamase (ESBL)/AmpC beta-lactamase (AmpC)-producing Enterobacteriaceae, posing a risk to public health.

Objectives
To investigate whether raw feed is a risk factor for household cats to shed ESBL-producing Enterobacteriaceae, a cohort study was designed. Additionally, raw and non-raw commercial pet food products were screened for the presence of ESBL-producing Enterobacteriaceae.

Methods
Weekly fecal samples of 17 cats in the control group and 19 cats in the exposed group were collected for three weeks and analyzed for the presence of ESBL-producing Enterobacteriaceae. Questionnaires were obtained to determine additional risk factors. Fecal samples were cultured on MacConkey agar supplemented with 1 mg/L cefotaxime. PCR and sequence analysis was used for screening for ESBL genes in suspected isolates. Pet food samples were cultured in LB broth supplemented with 1 mg/L cefotaxime and processed as described above.

Results
In the cohort study, ESBL-producing bacteria were isolated from 3 of 51 (5.9%) samples in the control group compared to 37 of 57 (89.5%) samples in the exposed group. A significant association was found between ESBL shedding and feeding raw pet food products (OR = 31.5). No other risk factors were identified in this study. ESBL-producing Enterobacteriaceae were isolated from 14 of 18 (77.8%) raw pet food products and 0 of 35 non-raw pet food products.

Conclusions
This study shows a strong association between shedding of ESBL-producing bacteria in household cats and feeding raw pet food. Raw pet food was often contaminated with ESBL-producing Enterobacteriaceae.

Submitter

Marcy Wilhelm-South

Purdue University

Publication Title PLoS One
Volume 12
Issue 11
Pages 11
DOI 10.1371/journal.pone.0187239
URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0187239
Cite this work

Researchers should cite this work as follows:

  • Valérie O. Baede; Els M. Broens; Mirlin P. Spaninks; Arjen J. Timmerman; Haitske Graveland; Jaap A. Wagenaar; Birgitta Duim; Joost Hordijk (2018), "Raw pet food as a risk factor for shedding of extended-spectrum beta-lactamaseproducing Enterobacteriaceae in household cats," https://habricentral.org/resources/63944.

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Tags
  1. Animal roles
  2. Bacteria
  3. Cats
  4. Feeds
  5. Mammals
  6. open access
  7. Pet ownership
  8. Pets and companion animals
Badges
  1. open access