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A modified HET-CAM approach for biocompatibility testing of medical devices

By C. Eder, E. Falkner, M. Mickel, C. Chiari-Grisar, H. Appl, H. Schoffl, S. Nehrer, U. M. Losert

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Abstract

The implantation of new biomedical devices into living animals without any previous toxicity or biocompatibility evaluation is possible under current legislation. The HET-CAM (Hen Egg Test-Chorionallantoic Membrane) test offers a partially immunodeficient, borderline in vitro/in vivo test system that allows the simulation of transplantation experiments to obtain biocompatibility data prior to animal testing. A collagen type I/III scaffold, designed for tissue regeneration, was tested for angiogenetic properties and biocompatibility patterns. A significant angiogenetic stimulus caused by the collagen scaffold material was observed. Altering biocompatibility patterns by incubation with the potentially hazardous chemicals acridine orange and ethidium bromide led to severe vessel thrombosis and a foreign body tissue response. CAM testing of biomaterials and tissue engineered products allows selection of the most suitable biomaterial and the elimination of unsuitable materials from animal experiments, leading to a refinement of testing procedures and a reduction in the number of animals required for biocompatibility testing.

Date 2005
Publication Title Animal Welfare
Volume 14
Issue 4
Pages 297-302
ISBN/ISSN 0962-7286
Language English
Author Address Department of Orthopaedic Surgery, Medical University Vienna, Vienna, Austria. eder@zet.or.at
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Tags
  1. Animal rights
  2. Animal welfare
  3. Compatibility
  4. Implantation
  5. Laboratory and experimental animals
  6. Laboratory animal science
  7. Medical research
  8. Methodologies
  9. peer-reviewed
  10. Research
  11. Techniques
  12. tests
  13. transplantation
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  1. peer-reviewed