Protein expression and genetic variability of canine Can f 1 in golden and Labrador retriever service dogs
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| Category | Journal Articles |
|---|---|
| Abstract |
Background: Valued for trainability in diverse tasks, dogs are the primary service animal used to assist individuals with disabilities. Despite their utility, many people in need of service dogs are sensitive to the primary dog allergen, Can f 1, encoded by the Lipocalin 1 gene (LCN1). Several organizations specifically breed service dogs to meet special needs and would like to reduce allergenic potential if possible. In this study, we evaluated the expression of Can f 1 protein and the inherent variability of LCN1 in two breeds used extensively as service dogs. Saliva samples from equal numbers of male and female Labrador retrievers (n=12), golden retrievers (n=12), and Labradorgolden crosses (n=12) were collected 1 h after the morning meal. Can f 1 protein concentrations in the saliva were measured by ELISA, and the LCN1 5′ and 3′ UTRs and exons sequenced. Results: There was no sex effect (p>0.2) nor time-of-day effect; however, Can f 1 protein levels varied by breed with Labrador retrievers being lower than golden retrievers (3.180.51 and 5.350.52 g/ml, respectively, p |
| Date | 2016 |
| Publication Title | Canine Genetics and Epidemiology |
| Volume | 3 |
| Issue | 3 |
| Pages | 8 |
| ISBN/ISSN | 2052-6687 |
| Publisher | Springer |
| DOI | doi.org/10.1186/s40575-016-0031-3 |
| URL | https://cgejournal.biomedcentral.com/articles/10.1186/s40575-016-0031-3 |
| Language | English |
| Author Address | Department of Animal Science, University of California, One Shields Ave, Davis, CA 95616, USA.amoberbauer@ucdavis.edu |
| Additional Language | English |
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