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Circadian Rhythm of Salivary Immunoglobulin A and Associations with Cortisol as A Stress Biomarker in Captive Asian Elephants (Elephas maximus)

By Tithipong Plangsangmas, Janine L. Brown, Chatchote Thitaram, Ayona Silva-Fletcher, Katie L. Edwards, Veerasak Punyapornwithaya, Patcharapa Towiboon, Chaleamchat Somgird

Category Journal Articles
Abstract

Salivary immunoglobulin A (sIgA) has been proposed as a potential indicator of welfare for various species, including Asian elephants, and may be related to adrenal cortisol responses. This study aimed to distinguish circadian rhythm effects on sIgA in male and female Asian elephants and compare patterns to those of salivary cortisol, information that could potentially have welfare implications. Subjects were captive elephants at an elephant camp in Chiang Mai province, Thailand (n = 5 males, 5 females). Salivette® kits were used to collect saliva from each elephant every 4 h from 06:00 to 22:00 h for 3 consecutive days (n = 15 samples/elephant). Enzyme immunoassays were used to quantify concentrations of IgA and cortisol in unextracted saliva. Circadian rhythm patterns were determined using a generalized least-squares method. Both sIgA and cortisol followed a circadian rhythm, although the patterns differed. sIgA displayed a daily quartic trend, whereas cortisol concentrations demonstrated a decreasing linear trend in concentrations throughout the day. There was no clear relationship between patterns of sIgA and salivary cortisol, implying that mechanisms of control and secretion differ. Results demonstrate for the first time that circadian rhythms affect sIgA, and concentrations follow a daily quartic pattern in Asian elephants, so standardizing time of collection is necessary. 

Submitter

Marcy Wilhelm-South

Purdue University

Date 2020
Publication Title Animals
Volume 10
Issue 1
Pages 13
Publisher MDPI
DOI 10.3390/ani10010157
URL https://www.mdpi.com/2076-2615/10/1/157
Language English
Additional Language English
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Tags
  1. Animal roles
  2. Animal welfare
  3. Elephants
  4. open access
  5. saliva
  6. Stress
  7. Zoo and captive wild animals
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  1. open access