In-tank underwater cameras can refine monitoring of laboratory fish
Contributor(s):: Ellis, T., Rimmer, G. S. E., Parker, S. J., Joiner, C., Sebire, M., Verner-Jeffreys, D. W., Lines, J.
Four- and six-hour urinary albumin excretion is a valuable alternative to 24-h urinary albumin excretion in male db/db mice
Contributor(s):: Norgaard, S. A., Sand, F. W., Sorensen, D. B., Sondergaard, H.
The ‘Cage Climber’ - A new enrichment for use in large-dimensioned mouse facilities
Contributor(s):: Vogt, Miriam Annika, Mertens, Sinja, Serba, Susanne, Palme, Rupert, Chourbaji, Sabine
Environmental Enrichment has been shown in experimental contexts to have clear and often beneficial effects on animal physiology and behavior. Housing prior to experiments can represent a large proportion of an animal’s living conditions, and improving housing conditions can be seen as...
The 3Rs and Humane Experimental Technique: Implementing Change
| Contributor(s):: Robert C. Hubrecht, Elizabeth Carter
In 1959, the Universities Federation for Animal Welfare (UFAW) Scholars Russell & Burch published the Principles of Humane Experimental Technique in which they laid out the principles of the Three Rs. However, the Three Rs owed much to others. It was UFAW and, in particular, UFAW’s...
Social Behavior of Adult Male New Zealand White Rabbits Housed in Groups or Pairs in the Laboratory
| Contributor(s):: Jr, Louis DiVincenti, Rehrig, Angelika
Rabbits are usually singly housed in laboratories, but a new emphasis on providing social housing for social species has prompted exploration of alternative housing for this species. However, a paucity of literature on the social behavior of rabbits in captivity has prevented scientific-based...
Effects of Metabolic Cage Housing on Rat Behavior and Performance in the Social Interaction Test
| Contributor(s):: Whittaker, Alexandra L., Lymn, Kerry A., Howarth, Gordon S.
Although the metabolic cage is commonly used for housing nonhuman animals in the laboratory, it has been recognized as constituting a unique stressor. Such an environment would be expected to affect behavioral change in animals housed therein. However, few studies have specifically addressed the...
Corticosterone Assimilation by a Voluntary Oral Administration in Palatable Food to Rats
| Contributor(s):: Raya, Juliana, Girardi, Carlos Eduardo Neves, Hipólide, Débora Cristina
Drug delivery in research on nonhuman animals in the laboratory is still challenging because it is usually invasive and stressful. Stress-free voluntary oral drug administration in water lacks precise control of dose and timing of substance ingestion. Voluntary oral consumption of corticosterone...
Impact of inhalation anaesthesia, surgery and analgesic treatment on home cage behaviour in laboratory mice
| Contributor(s):: Cesarovic, Nikola, Arras, Margarete, Jirkof, Paulin
Anaesthesia and analgesia are used frequently in laboratory routine to ensure animal welfare and good scientific outcomes in experiments that may elicit pain or require immobilisation of the animal. However, there is concern regarding the effect of these procedures on animal behaviour in...
Housing of female mice in a new environment and its influence on post-surgical behaviour and recovery
| Contributor(s):: Jirkof, Paulin, Cesarovic, Nikola, Rettich, Andreas, Arras, Margarete
The transportation of mice into a new clean cage after surgery is a standard procedure but might have detrimental effects during the critical post-surgical recovery phase. To analyse the effect of post-surgical housing, female C57BL/6J mice housed in their familiar home cage or in a new...
Behaviour, stress and welfare of Sprague Dawley rats (Rattus norvegicus) on diet board feeding for 24 months
| Contributor(s):: Laaksonen, Sakari, Nevalainen, Timo, Ketola, Jukka, Hau, Jann, Nieminen, Pentti, Haasio, Kristiina, Kasanen, Iiris, Voipio, Hanna-Marja
Diet board (DB) feeding aims to reduce the health hazards associated with ad libitum (AL) feeding. Rats have to gnaw wood to detach food from the DB, reducing their food consumption. We studied the welfare effects of DB by measuring faecal corticosterone metabolites (FCM), elevated plus-maze...
Buprenorphine via drinking water and combined oral-injection protocols for pain relief in mice
| Contributor(s):: Sauer, Mareike, Fleischmann, Thea, Lipiski, Miriam, Arras, Margarete, Jirkof, Paulin
Buprenorphine is the opioid analgesic most commonly used in laboratory mice. However, to maintain therapeutically effective serum levels, repeated injections are required. To overcome negative aspects of restraint and injection, oral self-administration is a promising alternative but has been...
Training success in group-housed long-tailed macaques (Macaca fascicularis) is better explained by personality than by social rank
| Contributor(s):: Wergård, Eva-Marie, Westlund, Karolina, Spångberg, Mats, Fredlund, Helene, Forkman, Björn
Using training to prepare laboratory animals for biomedical research is one important behavior management task. With increased knowledge about factors influencing training success, training programs may be optimized, resulting in a refinement of primate husbandry. Even when animals are trained...
An explorative study on the effect of provision of preferred and non-preferred enrichment on behavioural and physiological parameters in laboratory ferrets (Mustela putorius furo)
| Contributor(s):: Reijgwart, Marsinah L., Vinke, Claudia M., Hendriksen, Coenraad F. M., den Hoed, Kim M., van der Meer, Manon I., van der Meer, Miriam, Schoemaker, Nico J., van Zeeland, Yvonne R. A.
Environmental enrichment is often advocated to refine animal studies. Despite the increasing use of ferrets as an animal model in biomedical research, the knowledge on effects of the provision of enrichment on these animals is limited. Additionally, it is unknown whether varying types of...
Tramadol:Paracetamol in drinking water for treatment of post-surgical pain in laboratory mice
| Contributor(s):: Jirkof, Paulin, Arras, Margarete, Cesarovic, Nikola
In the search for stress-free analgesia administration for laboratory mice suffering pain, oral delivery of Tramadol:Paracetamol (T:P) shows great promise. Here, we monitored the analgesic efficacy and side effects of a T:P combination administered solely in the drinking water of female C57BL/6J...
To Group or Not to Group? Good Practice for Housing Male Laboratory Mice
| Contributor(s):: Sarah Kappel, Penny Hawkins, Michael T. Mendl
It is widely recommended to group-house male laboratory mice because they are ‘social animals’, but male mice do not naturally share territories and aggression can be a serious welfare problem. Even without aggression, not all animals within a group will be in a state of positive...
An ethical analysis of the 3 Rs
| Contributor(s):: Houde, Lisa, Dumas, Claude
Field testing, refinement, and psychometric evaluation of a new measure of quality of care for assisted living
| Contributor(s):: Rantz, Marilyn J., Aud, Myra A., Zwygart-Stauffacher, Mary, Mehr, David R., Petroski, Gregory F., Owen, Steven V., Madsen, Richard W., Flesner, Marcia, Conn, Vicki, Maas, Meridean